Abstract
Polyphenol oxidases (PPOs) are ubiquitous enzymes of plant defense which transform polyphenols into quinones that response to biotic and abiotic stresses. Transgenic Arabidopsis thaliana (L.) Heynh. lines harboring OsPPO promoter fused to β-glucoronidase (GUS) reporter gene, were treated exogenously with 0.1, 0.5 and 1 mM concentrations of salicylic acid (SA) and 5, 10 and 15 mM of hydrogen peroxide (H2O2). Plant samples were harvested after 1, 3 and 5 days of treatment. Transcriptional profiling of OsPPOGUS by qRT-PCR revealed the highest 12.96-fold induction with 0.5 mM SA after 3 days of treatment and the lowest fold induction (0.57) with 1 mM SA after one day. With H2O2, the OsPPOGUS mRNA transcript showed the highest accumulation of 21.08-fold change with 10 mM after 5 days of treatment and the lowest induction (3-fold) after 1 day of treatment by 15 mM H2O2. This specific OsPPO induction in response to SA and H2O2 clearly links OsPPO in potential responses to such stressors.
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