Abstract
Toxoplasmosis is an infection caused by Toxoplasma gondii , an intracellular obligate parasite. Its transmission is usually attributed to ingestion of undercooked or raw meat. The aim of this study was the detection and genotyping of T. gondii in meat products using the molecular method in East Azerbaijan. DNA was extracted from 164 meat products sample obtained from 15 commercial establishments. Nested polymerase chain reaction with specific primers for Toxoplasma gondii SAG2 locus was used for detection of the parasite in samples. Genotyping was carried out by digestion of PCR products with restriction enzymes Cfo1 and Sau3AI. T. gondii DNA was detected in 16.6, 19.1, 15 and 56.6%, in salami, sausages, and hamburger and kebab samples, respectively. Genotyping by restriction fragment length polymorphism (RFLP) analysis of SAG2 locus revealed that all of the samples belonged to genotype I. The detection of the parasite in uncooked meat and commercial meat products, and the high ratio of seropositive slaughtered animals, emphasis that the risk still exists for food -born toxoplasmosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
