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Abstract

Purpose: This study screened a collection of >2,800 naturally occurring products and identified Ignatius beans extract capable of inhibitingmTORC1 activity. HeLa cells were treated with aqueous extract from Ignatius beans to assess the activity of mTORC1. Treatment of HeLa cells with Ignatius bean extract inhibits the enzymatic activity of mTORC1 as assessed by the phosphoylration of p70 S6K (S6K) at Thr 308 in HeLa cells. This plant seed extract also exerts inhibitory effects on the activation phosphorylation of Akt. In addition, flowcytometry analysis revealed that Ignatius bean extract causes HeLa cells to accumulate inG2/Mphase of cell cycle. Trypanblue dye exclusion assay was carried out to determine the cytotoxicity of Ignatius Beans Methods: This plant seed extract also exerts inhibitory effects on the activation phosphorylation of Akt. In addition, flow cytometry analysis revealed that Ignatius bean extract causes HeLa cells to accumulate in G2/M phase of cell cycle. Trypan blue dye exclusion assay was carried out todetermine the cytotoxicity of Ignatius Beans. Results: This study has found that an aqueous extract from Ignatius beans inhibits mTORC1 activity as well as PI3K/Akt pathway resulting the accumulation of cell cycle at G2 to M phase in cultured human HeLa cells. This result suggests that the natural ingredient of Ignatius beans may directly inhibit mTORC1 activity or indirectly influencemTORC1 activity through the inhibition of Akt signaling. The inhibition of Akt phosphorylation at Thr308 strongly denies the involvement of negative feedback effect by PI3K/Akt pathway in cells treated with Ignatius bean extract. Conclusion: These data suggest that Ignatius bean extract could be used as a potent inhibitor of cell growth and cell proliferation. Contact: Boohyeong Byun, bhbyun@dhu.ac.kr