Abstract
Nude mice with orthotopic transplantation of human ovarian epithelial cancer were used to investigate screening criteria for paraneoplastic normal ovarian tissue and the security of the freezing and thawing for ovarian tissue transplantation. Expression of CK-7, CA125, P53, survivin, MMP-2/TIMP- 2 in paraneoplastic normal ovarian tissues were detected by RT-PCR as well as immunohistochemistry. The tissues of the groups with all negative indicators of RT-PCR, all negative indicators of immunohistochemistry, negative expression of CK-7, CA125 and survivin, positive expression of CK-7, CA125 and survivin, cancer tissues and normal ovarian tissues of nude mice were used for freezing and thawing transplantation, to analyze overt and occult carcinogenesis rates after transplantation. When all indicators or the main indicators, CK-7, CA125 and survivin, were negative, tumorigenesis did not occur after transplantation. In addition the occult carcinogenesis rate was lower than in the group with positive expression of CK-7, CA125 and survivin (P<0.01). After subcutaneous and orthotopic transplantation of ovarian tissues, rates did not change (P>0.05). There was no statistical significance among rates after transplantation of ovarian tissues which were obtained under different severity conditions (P>0.05). Negative expression of CK-7, CA125 and survivin can be treated as screening criteria for security of ovarian tissues for transplantation. Immunohistochemical methods can be used as the primary detection approach. Both subcutaneous and orthotopic transplantation are safe. The initial severity does not affect the carcinogenesis rate after tissue transplantation. Freezing and thawing ovarian tissue transplantation in nude mice with human epithelial ovarian carcinoma is feasible and safe.
Highlights
The treatments of ovarian cancer mainly include surgery and chemotherapy
Autologous transplantation of ovarian tissue has been used in a variety of malignant tumors to restore ovarian function, but there are several problems which limit the tentative application of autologous transplantation of ovarian tissues in epithelial ovarian cancer treatment
It was shown in this research that tumors of 15 cases were limited in ovaries and outside ovarian inoculation, including liver metastasis, intestines metastasis, ascites, retroperitoneal lymph node metastasis and wide range metastasis, of 25 cases happened in 40 ovarian orthotopic transplantation tumor models. 35 pieces of ovarian tissues which were detected to be normal by biopsy were obtained, which suggested that there was still a chance to obtain normal ovarian tissues for transplantation under the condition of wide range metastasis
Summary
The treatments of ovarian cancer mainly include surgery and chemotherapy. A considerable portion of patients with epithelial ovarian cancer are women of childbearing age. The loss of ovarian function will lead to a serial of clinical symptoms or metabolic syndrome (Michelsen et al, 2009). The common approach is hormone replacement therapy (HRT). Researches about the application of HRT to patients with epithelial ovarian cancer after surgery are rare. Its security has not been systematically certified by bulk of clinical researches. Clinical application is subject to certain restriction (MacLennan, 2011). It is necessary to find a more safe and effective way to solve the problem that patients with epithelial ovarian cancer lose ovarian function after surgery
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