Safety and properties of Enzymesporine microencapsulated probiotic product with enzyme and its effect on the physiology of intestinal digestion and weight gain of pigs

Abstract

The efficiency of the probiotic product Enzymesporine manufactured by Fermlab LLC was increased by the creation of its microencapsulated form with 5% crystalline trypsin, which surpassed its analogues in biological properties. In the microencapsulated probiotic product (MPP), the viability of the microorganisms was maintained at the level of the initial product by using 40% acetone as a precipitant. During in vitro experiments simulating gastric digestion, microcapsules were not destroyed for 120 minutes, which indicated their resistance to the acidic medium of the stomach and the possibility of passing it without loss of viability of the probiotic microflora. Tests in laboratory animals showed that the product did not have mutagenic activity and toxicity. During the use of this product from 48 days of life, reliable results were achieved in the increase of live weight and average daily gain in pigs of Genesus genetics at 98 and 148 days of life. By means of a qualitative caprological analysis, it was found that the product enhances the digesting ability in the intestines of pigs more intensively than its unencapsulated form without a proteolytic enzyme. It is recommended to use this product as a biologically active additive in the diet when feeding pigs of large industrial pig breeding.