Abstract
The results presented in this paper demonstrate the presence of an enzyme in extracts of an acetone powder of wheat germ which catalyzes the synthesis of S-methyl methionine from S-adenosyl methionine and methionine. The enzyme requires Zn ++ (10 −4 m) or Mn ++ (10 −3 m). The pH optimum for this reaction is approximately 6.5. The enzyme has been partially purified by (NH 4) 2SO 4 precipitation. When methionine- 14CH 3 is used as substrate, S-methyl methionine- 14C has been identified as a reaction product by co-chromatography in two solvents, by co-electrophoresis at one pH and by co-crystallization to constant specific activity with authentic unlabeled S-methyl methionine. S-Methyl methionine- 14C is also found as a product when either methionine- 14COOH or S-adenosyl methionine- 14CH 3 is introduced as the sole radiolabel. In addition, the synthesis of S-methyl methionine has been demonstrated with extracts of germinating mung beans and of barley shoots.
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