Saccharomyces jurei sp. nov., isolation and genetic identification of a novel yeast species from Quercus robur.

Samina Naseeb,Ian N Roberts,Beatrice Gini,Stephen A James,Carmen Nueno-Palop,Daniela Delneri,Christopher J Michaels,Christopher J Bond,Haya Alsammar,Henry Mcghie

doi:10.1099/ijsem.0.002013

Abstract

Two strains, D5088T and D5095, representing a novel yeast species belonging to the genus Saccharomyces were isolated from oak tree bark and surrounding soil located at an altitude of 1000 m above sea level in Saint Auban, France. Sequence analyses of the internal transcribed spacer (ITS) region and 26S rRNA D1/D2 domains indicated that the two strains were most closely related to Saccharomyces mikatae and Saccharomyces paradoxus. Genetic hybridization analyses showed that both strains are reproductively isolated from all other Saccharomyces species and, therefore, represent a distinct biological species. The species name Saccharomyces jurei sp. nov. is proposed to accommodate these two strains, with D5088T (=CBS 14759T=NCYC 3947T) designated as the type strain.

Highlights

The Saccharomyces sensu stricto group is composed of eight biologically distinct yeast species, namely Saccharomyces cerevisiae, S. paradoxus, S. cariocanus, S. uvarum, S. mikatae, S. kudriavzevii, S. arboricola and S. eubayanus [1,2,3,4,5,6], and two natural hybrids, namely S. pastorianus [7, 8] and S. bayanus [9]
Whilst the majority of isolated Saccharomyces were identified as S. paradoxus, two strains (D5088T and D5095) were recovered and found to represent a novel species belonging to the genus Saccharomyces
We show here that S. jurei is reproductively isolated from other Saccharomyces species by performing genetic crosses and testing for hybrid sterility

Summary

Introduction

The Saccharomyces sensu stricto group is composed of eight biologically distinct yeast species, namely Saccharomyces cerevisiae, S. paradoxus, S. cariocanus, S. uvarum, S. mikatae, S. kudriavzevii, S. arboricola and S. eubayanus [1,2,3,4,5,6], and two natural hybrids, namely S. pastorianus [7, 8] and S. bayanus [9]. Whilst the majority of isolated Saccharomyces were identified as S. paradoxus, two strains (D5088T and D5095) were recovered and found to represent a novel species belonging to the genus Saccharomyces. We show here that S. jurei is reproductively isolated from other Saccharomyces species by performing genetic crosses and testing for hybrid sterility.

Results

Conclusion