Abstract
Saccharomyces cerevisiae is widely applied as a probiotic in aquaculture activities, due to its ability to decontaminate the mycotoxin Aflatoxin B1 (AFB1). From this point, the present study aimed to evaluate the antifungal and anti-aflatoxigenic activities of inactivated Saccharomyces cerevisiae mixed with a commercial product (CP) of animal feed to assess its influence on the Aspergillus flavus and A. parasiticus fungi growth on the AFB1 production. Besides, AFB1 adsorption potential of S. cerevisiae and the commercial product was also investigated. Different concentrations of the commercial product alone and in the presence of inactivated yeast were analyzed by Aspergillus growth inhibition test, aflatoxin production by Aspergillus species, and AFB1 adsorption capacity. AFB1 detection and quantification were carried out by High-Performance Liquid Chromatography. The inactivated yeast and commercial product combination were effective in reducing A. flavus and A. parasiticus growth. A. flavusproduced less AFB1 after the inactivated yeast treatment, whereas A. parasiticus produced significantly less AFB1under a combination of inactivated yeast and 50% CP. Regarding AFB1 adsorption, 100% CP displayed the highest adsorption capacity at 10 ng mL-1 AFB1. At 25 ng mL-1 AFB1, only the treatment comprising inactivated yeast associated with 50% CP led to AFB1 adsorption, albeit at low levels. It was concluded that the association between the commercial additive and inactivated S. cerevisiae was effective in reducing the growth of fungi and there was AFB1 adsorption activity.
Highlights
Fish nutrition has advanced significantly with the expansion of aquaculture activities worldwide, aiming at improving performance indices to meet fish nutritional requirements
The mixture of commercial additive and inactivated yeast had a similar effect on A. flavus growth inhibition than that of the commercial product alone (p
The inactivated yeast associated with commercial additive, on the other hand, succeeded in inhibiting A. parasiticus growth (Figure 2)
Summary
Fish nutrition has advanced significantly with the expansion of aquaculture activities worldwide, aiming at improving performance indices to meet fish nutritional requirements. The yeast cell wall is a physical barrier that protects cells from environmental factors and is used as a biological mycotoxin adsorbent to reduce their gastrointestinal absorption during feeding (BOROVIKOVA et al, 2016; PEREYRA et al, 2018) In this scenario, Saccharomyces cerevisiae has been highlighted as a probiotic applied in fish farming, poultry farming, and pig farming, and its cell wall components have been shown to act as growth promoters and immune system stimulants, as well as mycotoxin adsorbents (ROSSI et al, 2010). Saccharomyces cerevisiae has been highlighted as a probiotic applied in fish farming, poultry farming, and pig farming, and its cell wall components have been shown to act as growth promoters and immune system stimulants, as well as mycotoxin adsorbents (ROSSI et al, 2010) In this context, the present study aimed to evaluate the antifungal and anti-aflatoxigenic activities of inactivated Saccharomyces cerevisiae, both isolated and mixed with a commercial product, and determine its effects on Aspergillus flavus and Aspergillus parasiticus growth and Aflatoxin B1 (AFB1) production.
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