Year-end Sale % OFF 
Abstract
Background DNA vaccines are a good option to generate a desired antigen using the cellular machinery of the vaccinated subject. This kind of vaccine can induce both of the humoral and cellular immune response, and show high stability and easy working, as well as offer a low-cost production. and safety for immune compromised patients [1]. However, some disadvantages, such as the low transfection rate and low immunogenicity, makes necessary the use of multiple doses [2,3]. Therefore, several studies have been conducted trying to increase the immune response generated by the DNA vaccines. Sacharomyces boulardii, a probiotic yeast that is capable to increase the host immune response [4], was not previously evaluated as an adjuvant for DNA vaccines. Thereby, in the present study we evaluated the S. boulardii capacity to increase the humoral response of mice using DNA vaccines against a fragment of the leptospiral antigen LigAcloned in the mammalian expression plasmid pTARGET [4,5].
Highlights
DNA vaccines are a good option to generate a desired antigen using the cellular machinery of the vaccinated subject
Blood samples were collected from the retro eye venous plexus of the animals on days 0, 13, 20 and 27 for the humoral immune response evaluation of each immunization through indirect ELISA, using recombinant LigAni (5 μg.mL-1) as antigen and serum dilutions 1:30 in PBS-T.ELISA units were calculated by dividing the mean absorbance of each group by the mean absorbance of the day 0
There was nosignificant difference between the second and third immunizations of group 2 (G2). This way, the results indicate that using S. boulardii as adjuvant, only two immunizations are sufficient to obtain an adequate immune response
Summary
DNA vaccines are a good option to generate a desired antigen using the cellular machinery of the vaccinated subject. Material and methods Four to six month-old Female BALB/c mice were separated into 2 groups of 12 animals each.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
