Abstract
The effects of probiotics on blood glucose and cardiovascular complications induced by diabetes have been inconsistent. Therefore, the aim was to assess the effect of Saccharomyces boulardii (Sb) on metabolic and cardiovascular dysfunction in streptozotocin (STZ)-induced type-1-diabetes (STZ-T1D). Male C57BL/6 mice were randomized into: control (C), diabetes (D), control+Sb (CSb) and diabetes+Sb (DSb) (n=6-12/group). Mice were made diabetic with STZ (150mg/kg) and daily treated with Sb (0,5x108 CFU) by oral gavage. After 8 weeks of treatment, blood pressure (BP) and heart rate (HR) signals obtained from conscious mice were recorded. Spectral analysis of systolic BP (BPV) and HR variabilities (HRV) was performed to estimate autonomic modulation to the heart and vessels. Plasma was collected and heart was excised for biochemical analyses. D mice presented metabolic dysfunction, with increased blood glucose, triglycerides and cholesterol, as compared with C animals. On the other hand, treatment with Sb reduced blood glucose (DSb=238±39 vs. D=378±17 mg/dl) and triglycerides (DSb=121±11 vs. D=169±10 mg/dl), with no effect on cholesterol levels (DSb=119±4 vs. D=106±9 mg/dl). Probiotic treatment also reduced cardiac IL-6 (DSb=80±8 vs. D=113±13 pg/mg) and increased IL-10 (DSb=251±16 vs. D=185±11 pg/mg), leading to a beneficial impact on heart nitric oxide levels (DSb=12.4±1.3 vs. D=7.6±0.9 pg/mg). D mice presented a significant reduction in HRV, BPV and also in low-frequency component of systolic BP (SBP). Treatment of D mice with Sb increased HRV (DSb=20±0.9 vs. D=10±0.9 ms²), BPV (DSb=8±0.4 vs. D=5±0.3 mmHg²) and low-frequency component of SBP (DSb=98±8 vs. D=55±8 mmHg²). Our study shows, for the first time, that administration of Sb is strongly associated with glycemic control, cardiovascular protection and improvement of inflammatory profile in STZ-T1D. Disclosure A.B.P. Brandão: None. I.C. de Abreu: None. F. Aimbire: None. E.M. Higa: None. A. Casali: None. F.G. Ferreira: None. R.M. Albuquerque: None. L.B. Santos: None. M.C. Irigoyen: None. K.R. Casali: None. T.S. Cunha: None.
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