Year-end Sale % OFF 
Abstract
The use of interspecific hybrids during the industrial fermentation process has been well established, positioning the frontier of advancement in brewing to capitalize on the potential of Saccharomyces hybridization. Interspecific yeast hybrids used in modern monoculture inoculations benefit from a wide range of volatile metabolites that broaden the organoleptic complexity. This is the first report of sake brewing by Saccharomyces arboricola and its hybrids. S. arboricola x S. cerevisiae direct-mating generated cryotolerant interspecific hybrids which increased yields of ethanol and ethyl hexanoate compared to parental strains, important flavor attributes of fine Japanese ginjo sake rice wine. Hierarchical clustering heatmapping with principal component analysis for metabolic profiling was used in finding low levels of endogenous amino/organic acids clustered S. arboricola apart from the S. cerevisiae industrial strains. In sake fermentations, hybrid strains showed a mosaic profile of parental strains, while metabolic analysis suggested S. arboricola had a lower amino acid net uptake than S. cerevisiae. Additionally, this research found an increase in ethanolic fermentation from pyruvate and increased sulfur metabolism. Together, these results suggest S. arboricola is poised for in-depth metabolomic exploration in sake fermentation.
Highlights
As currently defined, the budding yeast clade known as Saccharomyces has eight members: S. cerevisiae, S. paradoxus
Limited research has been conducted on the metabolic capabilities of S. arboricola and these results suggest S. arboricola and the hybrids’ propensity for fermentation may be suited for sake production
This study explored the novel use of S. arboricola in Japanese rice wine known as sake, traditionally produced from polished rice with A. oryzae during winter months
Summary
The budding yeast clade known as Saccharomyces has eight members: S. cerevisiae, S. paradoxus Uvarum), S. mikatae, S. kudriavzevii, S. arboricola S. arboricolus), S. eubayanus, and S. jurei [1,2,3,4,5,6,7,8,9]. S. carlsbergensis, S. monacensis) and S. bayanus are two natural hybrids found in this group [10,11,12]. S. arboricola was first isolated from the tree bark of the Fagaceea family in the west and southwest of China [2] in 2008 and later isolated in western
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
