Saccharomonospora xiaoerkulensis sp. nov., isolated from lake sediment.

Abstract

A novel actinomycete, strain TRM 41495T, was isolated from lake sediment of Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41495T was aerobic, Gram-stain-positive, growing best on ISP medium 7 with abundant white aerial mycelium formed and brown soluble pigments produced. The optimum NaCl concentration for growth was 3.0 % (w/v), and the optimum temperature for growth was 37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41495T was shown to belong to the genus Saccharomonospora with a sequence similarity of 97.50 % with the most closely related species, Saccharomonospora xinjiangensis. The organism had chemical and morphological features typical of the genus Saccharomonospora. The cell wall of strain TRM 41495T contained meso-diaminopimelic acid, and galactose, arabinose, ribose and mannose were the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and an unknown phosphoglycolipid. The predominant menaquinones were MK-9(H4) and MK-9(H2). The major fatty acids were iso-C16 : 0, C16 : 0, C17 : 1ω6c, anteiso-C17 : 0 and iso-C16 : 1H. The G+C content of the DNA was 72.9 mol%. However, DNA-DNA hybridization studies between strain TRM 41495T and Saccharomonospora xinjiangensis showed only 43.9 % relatedness. Whole-cell sugars, menaquinones and fatty acids differentiate strain TRM 41495T from Saccharomonospora xinjiangensis. On the basis of the polyphasic evidence, strain TRM 41495T represents a novel species of the genus Saccharomonospora, for which the name Saccharomonospora xiaoerkulensis sp. nov. is proposed. The type strain is TRM 41495T (=CCTCC AA 2015038T=KCTC 39727T).