Saccharification of sugarcane bagasse using cellulases and oxidoreductases enzymes

Abstract

Lignin is the major polymer responsible for limiting the rates of lignocellulose degradation. Enzymatic hydrolysis of lignin (using oxidorreductases enzymes) open the way to action of cellulose and hemicellulose -degrading enzymes systems to obtain a fermentable hydrolysate rich in monomeric compounds from the carbohydrate polymers present in the biomass. Trichoderma reesei is an important fungus that is widely used as a source of cellulases for the hydrolysis of plant cell wall polysaccharides. In this study, was identified the filamentous fungi Pestalotiopsis sp BBF245, as a promising strain for production of oxidoreductases enzymes. Enzyme cocktails were composed, using cellulases from T. reesei and oxidoreductases enzymes from Pestalotiopsis sp BBF245 for hydrolysis of sugarcane bagasse (SCB) in natura. The amounts of reducing sugars released from the saccharification of bagasse was of 15,52 g/L after 24 hours of incubation. The results show that the combination of lignocellulose-degrading enzymes significantly increased the degradation of sugarcane bagasse and suggest that the use of enzyme cocktails oxidoreductases and cellulases may significantly improve the hydrolysis of biomass.