Sa1941 The Morphological Features of Colorectal Tumors That Are Associated With the Appearance of Methylated DNA in Plasma

Abstract

It is recently reported that microRNAs (miRNAs) are stably present in serum and potentially useful in the diagnosis of cancer. Using highly sensitive microarray, 3D-Gene®, we examined comprehensive miRNA expression profiles in serum obtained from 66 pancreatic cancer patients, 66 bile duct cancer patients and 100 matched healthy volunteer as the study set, and found that 122 miRNA markers for pancreatic cancer and 125 miRNA markers for bile duct cancer differentiated pancreatic / bile duct cancer patients from healthy control with a statistical significance. We then validated the diagnostic performance of these markers using serum obtained from 33 pancreatic cancer patients, 33 bile duct cancer patients and 50 healthy controls. One of the single miRNA markers for pancreatic cancer yielded more than 90% diagnostic sensitivity and specificity, and combined use of two markers perfectly differentiated cancer and healthy samples. We also found that over 100 miRNA markers of pancreatic cancer overlapped with miRNA markers of bile duct cancer. Our results suggest that these miRNA markers have clinical values to screen out pancreatic and bile duct cancer patients.