Sa1181 Abnormalities of Colonic Motilities Play a Crucial Role in Symptom Onset for Irritable Bowel Syndrome With Diarrhea

Abstract

volunteers (HV) and 12 IBS patients and responses to exogenously applied capsaicin (0.1, 1 and 10 nM) and histamine (1, 10 and 100 μM) were monitored using calcium (Ca2+)imaging. In addition, the effect of 10 μM histamine pre-incubation on the capsaicin response was studied in HV. In parallel, murine dorsal root ganglia (DRG) were isolated to study the calcium response to capsaicin (10 250 nM) in the presence of histamine or after overnight incubation with mucosal biopsy supernatant from HV and IBS patients. Pyrilamine (1 μM) was used as H1R antagonist. Results: Application of histamine and capsaicin evoked significantly higher peak amplitudes in submucosal neurons from IBS patients compared to HV (Table 1). Pretreatment with histamine significantly increased the peak amplitudes in response to capsaicin in submucosal neurons from HV (Table 1). This sensitization of TRPV1 by histamine was confirmed in mouse DRG neurons, an effect that was prevented by preincubation with pyrilamine (Table 2). In parallel studies, overnight incubation with HV supernatant spiked with 10 μM histamine significantly increased the response to capsaicin compared to control HV supernatant. Similarly, overnight exposure of DRG neurons to IBS supernatant significantly increased the capsaicin response, an effect that was reduced by pyrilamine (Table 2). Conclusion: We showed that submucosal neurons of IBS patients are more intensely activated by capsaicin than those of HV. This effect can be mimicked by preincubation with histamine in both human submucosal neurons and mouse DRG neurons. Furthermore, incubation of mouse DRG neurons with IBS supernatant also increases the response to TRPV1, an effect that is blocked by the H1R antagonist pyrilamine. Based on these data, we conclude that histamine sensitizes TRPV1 on sensory neurons, most likely contributing to increased visceral pain perception in IBS. Moreover, we hypothesize that the therapeutic effect of the H1R antagonist ebastine in IBS patients can at least partly be explained by interference with this pathway. Table 1 Submucosal neuronal activation in rectal biopsies (% response)