Abstract

PCR-restriction fragment length polymorphism and the Genescan analyses: GT microsatellite polymorphism in the TLR2 gene, Asp299Gly in the TLR4 gene, -260C/T and -550C/T polymorphisms in the CD14 gene. For the TLR2 GT microsatellite polymorphism, we classified alleles into two subclasses, 12-16 GT repeats (S allele) and 17-28 repeats (L allele). Statistical analysis was performed using the Fisher's exact test. Results : The frequencies of the S allele of the TLR2 GT microsatellite polymorphism, the T allele of -260 C/T, and C allele of CD14 -550 C/T were higher in patients with AP (p=0.0006 for the S allele TLR2 GT polymorphism, p = 0.0147 for the -260 T allele, and p = 0.0292 for -550 C allele). No TLR4 Asp299Gly polymorphism was found. The frequency of S allele in the TLR2 GT microsatellite polymorphism was higher in patients with severe AP than those with mild AP. For the other polymorphisms, no differences were observed in subgroups based on severity or etiology of pancreatitis. None of the polymorphisms examined showed significant difference in the distribution of genotypes or alleles between the patients with and without bacterial infection. Conclusions : TLR2 intron 2 GT repeats, CD14 -260 C/T and CD14 -550 C/T polymorphism are associated with AP in Japan.

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