S2069 Lactose and Fructose Malabsorption in Patients with Functional Bowel Disorders

Abstract

G A A b st ra ct s University Hospital, were enrolled. Patients with symptoms consistent with IBS diarrhea and healthy control were included. History and physical examination was done. Stool examination was done by microscopy, culture for B hominis and polymerase chain reaction (PCR) for B. hominis and its typing was also done. DNA extraction from stool culture was done with stool DNA extraction kit (Qiagen). Primers used for PCRwere previously described. Two sided Fischer-exact test was used to determine the statistical significance. Results: There were thirty-three patents with mean age 43±17.2, age range 16-75 years and male; female ratio of 24:9. Patients with IBS-diarrhea were 27(82%) and healthy control 6(18%). Twentyone (64%) had infection with a single type of B hominis, 6(18%) had mixed type while 6(18%) were not typeable. Infection with B hominis type 1 was seen in 13(39%), type 3 in 8(24%) and mixed type in 6(18%). Of twenty-three patients with IBS-diarrhea 17(74%) had infection with single type of B hominis while 6(26%) with mixed types as compared with control 4(100%) having single type of B hominis p= 0.54. Conclusion: Patients with IBS-diarrhea had infection with single genotype of B hominis, predominantly type 1. However, these are the preliminary results of an ongoing study.