Abstract
Infectious Bronchitis virus (IBV) causes acute and contagious diseases in chickens with significant impact on the poultry industry worldwide. The objective of this study is to develop DNA and subunit vaccines containing S1, M or N protein of infectious bronchitis virus (IBV) to protect chickens against IBV challenge. The individual S1, M or N gene was constructed into pTriEx vector as pTriEx-3/S1, pTriEx-3/M and pTriEx-3/N, and transformed into E. coli for plasmid preparation or protein expression. All the DNA and subunit vaccines were confirmed by immunefluorescence assay and Western blotting, respectively. Chickens were vaccinated from one week of age by intramuscular injection weekly three times for DNA vaccines and two times for subunit vaccines. Chickens were challenged by IBV and observed for one or two weeks. All sera were collected weekly and detected by commercial IBV ELISA kit. Chickens were sacrificed and kidney lesion score was given to each chicken according to the severity of damage and swelling in kidney. Chickens vaccinated with N DNA vaccines had significantly higher anti-IBV ELISA titers than those vaccinated with M and S1 DNA vaccines (p 0.05). Chickens vaccinated with pTriEx-3/S1 DNA/subunit vaccines and pTriEx-3/M DNA vaccine did not have significantly different ELISA titers than those in negative control before challenge and did not have significantly different lesion scores than those in challenge control, either. Chickens vaccinated with M or N subunit vaccines had significantly higher anti-IBV ELISA titers than those in other groups (p 0.05). The results showed that M and N subunit vaccines could evoke immune response and provide protection against IBV challenge.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
