Abstract
Nuclear genes encoding plastid ribosomal proteins are more highly expressed in leaves than in roots. This leaf-specific induction seems to be light-independent. We have previously characterized a spinach nuclear factor S1F binding to a cis-element within the rps1 promoter, which negatively regulates both the rps1 and the cauliflower mosaic virus 35S promoters in transient expression assays. Here, we show that the S1F binding site is related to but different from the light-responsive Box II of the pea rbcS-3A promoter, which is recognized by the nuclear factor GT-1. Transgenic plant analyses showed that the S1F site tissue-specifically represses the rps1 promoter in roots as well as in etiolated seedlings. We suggest that the GT-1-related S1F binding site is responsible, at least in part, for the transcriptional repression of rps1 in nonphotosynthetic tissues such as roots.
Highlights
S1F Binding SiteIs Related to but Differentfrom the Light-responsive GT-1 Binding Site and Differentially Represses the Spinach rpsl Promoter in Transgenic Tobacco*
We suggest that the GT-1-related S1F bindingsite we investigated further the relationship between these binding is responsible, at least in part, for the transcriptional sites and the GT-1 binding site
Sites 1 a n d 3 of the rpsl Promoter Are Tho Related Binding Sites-Examination of the nucleotide sequences of the three sites of the rpsl promoter (Zhou et al, 1992) showed that they are all rich in nucleotide T, A, and G in either the uppeorr the lower strand (Fig. 1).This similarity prompted us to examine whether they are relateDdNA binding sites.In the firesxt periment, dimers of Site 1were 32P-labeledand used as probe in competition binding assays
Summary
0 1994 by The American Society for Biochemistry a n d Molecular Biology, Inc. Vol 269, No 24, Issue of June 17, pp. 16626-16630, 1994 Printed in U.S.A. 0 1994 by The American Society for Biochemistry a n d Molecular Biology, Inc. Vol 269, No 24, Issue of June 17, pp. 16626-16630, 1994 Printed in U.S.A. S1F Binding SiteIs Related to but Differentfrom the Light-responsive GT-1 Binding Site and Differentially Represses the Spinach rpsl Promoter in Transgenic Tobacco*. (Received for publication, January 12, 1994, and in revised form, March 14, 1994). From the Laboratoire de Biologie Mole'culaire Ve'ge'tale, Centre National de la Recherche Scientifique,Unite' de Recherche Associe'e 1178, Universite' Joseph Fouriel; F-38041Grenoble Ce'de'x,France
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