Abstract
S100A12 belongs to the S100 family and acts as a vital regulator in different types of tumors. However, the function of S100A12 in thyroid carcinoma has not yet been investigated. In this study, we analyzed the expression of S100A12 in human papillary thyroid cancer (PTC) samples and two PTC cell lines. In addition, we explored the effects of S100A12 on PTC cell progression in vitro and in vivo. Our results showed that S100A12 was significantly upregulated in PTC specimens. Moreover, silencing S100A12 markedly inhibited PTC cell proliferation, migration, invasion and cell cycle progression. In addition, knockdown of S100A12 significantly reduced the expression of CyclinD1, CDK4 and p-ERK in PTC cells. An in vivo study also showed that silencing S100A12 dramatically suppressed tumor cell growth and decreased Ki67 expression in a xenograft mouse model. This study provides novel evidence that S100A12 serves as an oncogene in PTC. Knockdown of S100A12 suppressed PTC cell proliferation, migration, and invasion and induced G0/G1 phase arrest via the inhibition of the ERK signaling pathway. Therefore, S100A12 may be a potent therapeutic target for PTC.
Highlights
S100A12 belongs to the S100 family and acts as a vital regulator in different types of tumors
The S100 proteins family is a group of calcium-binding proteins that is encoded by chromosome 1q21 and contains over 20 members with comparable amino acid structures[6]
It has been demonstrated that S100A12 is extensively expressed in the mucosa of oropharyngeal squamous cell carcinoma (OPSCC)and serves as a prognostic factor[19]
Summary
S100A12 belongs to the S100 family and acts as a vital regulator in different types of tumors. Knockdown of S100A12 suppressed PTC cell proliferation, migration, and invasion and induced G0/G1 phase arrest via the inhibition of the ERK signaling pathway. Previous studies suggested that the S100 family exerts a crucial effects on the growth and metastasis of tumors by regulating the oncogenic microenvironment[6,8,9]. S100A12 is expressed in multiple tumor cells, and contributes to mediating various vital cellular functions, including proliferation17,invasion, and migration[18]. S100A12 has been indicated to suppress proinflammatory and anti-inflammatory cytokines, suggesting that S100A12 could regulate proinflammatory and anti-inflammatory cytokines through the ERK signaling pathway activated[27], which is important in PTC in this study, to investigate the contribution of S100A12, we examined its expression in human PTC samples by immunohistochemistry and western blotting. Explored its role in PTC cell proliferation migration, and invasion in vitro and in vivo
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