Abstract
Ascaris suum is a soil-transmitted parasite causing ascariasis in pigs, largely limiting livestock production globally. Searching for new drugs affecting all stages of nematode development is necessary and widely postulated. The in vitro activity of S-methyl-(2-methoxycarbonylamino-benzoimidasole-5) thiosulfonate on A. suum developing eggs was studied. Five concentrations of the drug were used—0.625, 1.25, 2.5, 5 and 10 mM during 24, 48 and 72 h of exposure. After drug treatment, the eggs were washed and cultured in 0.05 M HCl at 27 °C for 20 days. Both the concentration and duration of the drug exposure had an inhibitory impact on the percentage of L2 larvae developed. The best effect was obtained after 72 h of incubation in 5 mM drug solution, only 1.9 ± 3.3% of the larvae developed to the L2 stage. Moreover, no SNP was detected at codon 167, which is correlated with benzimidazole resistance, in the tested samples. For the first time, it has been demonstrated that S-M-(2-MKA-BZ-5)TS seems to be a potential ovicidal anti-helminthic agent. It may lead to the elimination of parasites and reduce environmental contamination from roundworm eggs. The ovicidal effects of the drug should be additionally confirmed by further infection studies using experimental animals.
Highlights
Ascaris suum Goeze, 1782, is the most widespread intestinal parasite causing ascariasis in pigs
The best effect was obtained after 72 h of incubation in 5 mM drug solution, only 1.9 ± 3.3% of the larvae developed to the L2 stage
No single nucleotide polymorphism (SNP) was detected at codon 167, which is correlated with benzimidazole resistance, in the tested samples
Summary
Ascaris suum Goeze, 1782, is the most widespread intestinal parasite causing ascariasis in pigs. This soil-borne infection has a high worldwide prevalence and causes significant economic losses. It manifests by reducing the swine production efficiency and condemnation to organs such as the liver [1]. A. suum is often postulated as a model for research on human infection by Ascaris lumbricoides Linnaeus, 1758. Human ascariasis affects approximately 820 million people, most often the younger generations, especially in areas with poor access to water, sanitation and hygiene [2,3,4,5]. Benzimidazoles (BZ), imidazothiazoles/tetrahydropyrimidines (LV) and the macrocyclic lactones (ML)
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