Ruthenium(II) carbonyl complexes containing thiourea ligand: Enhancing the biological assets through biomolecules interaction and enzyme mimetic activities

Paranthaman Vijayan,Subbarayan Vijayapritha,Wolfgang Linert,Periasamy Viswanathamurthi,Chidambaram Ruba

doi:10.1007/s00706-019-2357-5

Paranthaman Vijayan, Subbarayan Vijayapritha + Show 3 more

Open Access

https://doi.org/10.1007/s00706-019-2357-5

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Abstract

The ligand H2L (N-(N,N-diethylaminothiocarbonyl)benzimidoylchloride-2-aminoacetophenone-N-methylthiosemicarbazone) reacts with ruthenium(II) building blocks [RuHCl(CO)(EPh3)3] (E = P or As) to form new complexes [Ru(1,1-DT)(Cl2)(CO)-(EPh3)2] (E = P or As; 1,1-DT = 1,1-diethylthiourea). The ligand H2L in these reactions undergoes C=N bond break and coordinates through sulfur atom of C=S group. Analytical and spectral (IR, UV–Vis, NMR, ESI-MS) methods were used to characterize the compounds. A distorted octahedral geometry for complexes has been furnished by X-ray crystallography, which confirmed the coordination mode of the ligand with metal precursor. The binding affinity and mode of binding of the complex towards some important biomolecules such as calf thymus DNA and bovine serum albumin protein were determined using absorption and emission spectra and found intercalative binding with calf thymus DNA and static interaction with bovine serum albumin. The in vitro cytotoxic activity of complex was assessed using human cervical cancer (HeLa), human hepatocellular carcinoma (HepG2), and normal Vero cells. Furthermore, the complex was found to possess significant enzyme mimic catalytic activity in oxidation and hydrolysis reactions.Graphical abstract

Highlights

In recent years, attention has been given for metallic drug–DNA interaction in inorganic pharmaceutical research
The ligand H 2L was obtained by simple condensation using our previously reported methodology [18] with crystalline purity and sufficient yield to use without further purification for the complex synthesis
As a basic testing method, emissive titration study is unanimously employed method to find out the binding mode of metal complexes with DNA which usually involves the changes in emissive intensity and wavelength

Summary

Introduction

Attention has been given for metallic drug–DNA interaction in inorganic pharmaceutical research. Keywords Thiourea · Ruthenium(II) complex · DNA/BSA binding · Enzyme kinetics As a basic testing method, emissive titration study is unanimously employed method to find out the binding mode of metal complexes with DNA which usually involves the changes in emissive intensity and wavelength.

Results

Conclusion