Abstract
bacterial strains with lipase activity were separated from the waste water of the meat- processing enterprises and Volgograd water-handling facilities aeration tank. There were studied their morphological, cultural and biochemical properties and their animal and vegetable fat splitting ability. Comparative analysis of fat splitting ability has been carried out for searched strains and devices of enzyme activity increasing and growing process intensity of searched microorganisms were investigated. Mineral nature ingredients - the lake Elton brine, the bischofite, the Dead Sea salt - were added in the culture mediums for increasing of biomass accumulation. It has been investigated that addition of the brine into the culture medium in 5 % concentration increases the growth rate of producer lipase on 293 % in comparison with growth of the control medium without the brine. Addition of 0,25 % the Dead Sea salt concentration intensifies the growth on 317 % in comparison with the control medium. Laboratory simulation of fat waste water purification process has been realized with one of separated activity bacterial strains and it has been showed that addition of the 0,5 % bishofite into the fermenter stimulates lipase activity of the searched microorganisms.
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