Abstract
The rupture force changes to separate the third strand from the duplex within an oligonucleotide-directed triplex (triplex DNA) were measured in the presence of small DNA binding molecules by means of atomic force spectroscopy. Rupture force histograms revealed a rupture force of 44.3±0.4pN to separate the third strand from duplex DNA in pure buffer solution at a rupture velocity of 400nm∕s. In 30μM of Ru(bipy)2(dppz)2+ buffer solution an increase of rupture force to 57±2.2pN was observed. For 30μM Ru(phen)2(dppz)2+ a rupture force of 65.1±3.9pN was determined. These results show that both intercalative molecules increase the stability of triplex DNA significantly.
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