Abstract
Several published reports have indicated that derangement of the phosphocreatine/creatine (Cr) energy-buffering system via Cr analogue feeding results in cardiomyopathy when cardiac performance is assessed in vitro. The present study was designed to examine indexes of cardiac performance in rats that have been chronically Cr depleted. Adult (180 +/- 4 g) rats were assigned to a normal diet (ND) (n = 8) or a Cr-depletion diet (CD) group (n = 10). After 61 +/- 1 days of ad libitum feeding, measurements of steady-state exercise O2 consumption were made. Hemodynamic indexes were then assessed during incremental running to peak sustained levels. Rats were then killed and the left ventricle was excised. In the CD group Cr was depleted 82% and V1 isomyosin decreased while V2 increased. O2 consumption during steady-state running was not different in CD rats. The respiratory exchange ratios of CD rats reflected a bias toward fat utilization during the latter stages of prolonged exercise. The exercise heart rates and peak systolic blood pressures of CD rats were slightly lower than those of ND rats. Both negative and positive rates of left ventricular pressure development were significantly reduced at all running speeds in the CD rats. CD rats were capable of exercise performance equal to that of ND animals. The hemodynamic and metabolic data suggest that the adaptations seen in the CD animals may be similar to those reported after endurance training. These results indicate that chronic Cr depletion does not impair either the circulatory or exercise capacity of rodents.
Highlights
The findings reported clearly suggest that peak exercise capacity and cardiac performance is not compromised in Cr-depletion diet (CD) rats
In agreement with several isolated heart studies, the hearts of CD rats in the present study did exhibit altered cardiac performance parameters
Similar results have been reported after endurance training in rats [6]
Summary
Experimental design. ,B-Guanidinopropionic acid (/3-GPA), a slowly metabolized analogue of Cr, was synthesized from ,8-alanine and cyanamide The respiratory exchange ratio (RER) was calculated for each time block The purpose of these measurements was to determine whether the metabolic profiles of control and CD rats were different as reflected by changes in Vo2 or RER during a steady-state period of increased metabolic load. Five-microliter aliquots of stored myofibril solution were added to 35 μl of denaturing buffer (62.5 m.t'\,f tris(hydroxymethyl)aminomethane, 20% glycerol, 1% ,8-mercaptoethanol, 2.3% sodium dodecyl sulfate (SDS), and 0.05% bromophenol blue) and heated at 100°C for 2 min. After this treatment, a 16-μl aliquot (2 μg) was TABLE 1. Percent data presented in tables and figures represent the nontransformed values
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
