Abstract
A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.
Highlights
Strawberry (Fragaria x ananassaDuch) is a fruit that originally grown in subtropical area, it is cultivated in tropical country, such as Indonesia
The condition is in accordance with Dijkstra (1993), Swartz et al (1981), Nehra et al (1994) which suggested that using runners as planting materials for strawberry is not suitable because of their sensitivity to pathology agents
Strawberry (Fragaria x ananassa Duch) variety of Rosalinda that was taken from an orchard in Pancasari, Buleleng Regency, Bali (Indonesia) was used as source of mother plants
Summary
Strawberry (Fragaria x ananassaDuch) is a fruit that originally grown in subtropical area, it is cultivated in tropical country, such as Indonesia. Pancasari is a sub district in Buleleng Regency, Bali, Indonesia with an altitude of ≥ 1100m above sea level, an area where strawberry is cultivated intensively. The farmers obtain the planting materials for beginning plantation from tissue culturepropagated plants which a relatively expensive due to come from outside Bali. They reported that using runners as planted materials for subsequent plantation lead to a decrease yield due to pathogen attack The condition is in accordance with Dijkstra (1993), Swartz et al (1981), Nehra et al (1994) which suggested that using runners as planting materials for strawberry is not suitable because of their sensitivity to pathology agents. Moradi et al (2011) proposed that micropropagated strawberry plants have been introduced to
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