Runella salmonicolor sp. nov. and Dyella lutea sp. nov., isolated from paddy field soil.

Abstract

Bacterial strains were collected from the soil of a paddy field around Dongguk University in Goyang, Republic of Korea. Two Gram-stain-negative, rod-shaped, aerobic or facultatively anaerobic bacterial strains were designated S5T and SaT. The results of analysis of phylogenetic trees based on 16S rRNA and whole-genome sequences indicated that these two strains represented a member of the genus Runella and a member of the genus Dyella, respectively. S5T exhibited 99.22, 98.10 and 97.68 % similarity to Runella rosea HYN0085T, Runella aurantiaca YX9T and Runella slithyformis DSM 19594T, respectively. S5T grew at 15-40 °C (optimum, 25 °C), at pH 6.5-12.0 (optimum, pH 9.5) and in the presence of 0-0.5 % (w/v) NaCl (optimum, 0 %). SaT exhibited 99.18 %, 98.36 %, 97.82 % and 97.68 % similarity to Dyella thiooxydans ATSB10T, Frateruia defendens DHoT, Fulvimonas yonginensis 5HGs31-2T and Dyella ginsengisoli Gsoil 3046T, respectively, and grew at 20-40 °C (optimum, 30 °C), at pH 5.5-11.0 (optimum, pH 8) and in the presence of 0-4.5 % (w/v) NaCl (optimum, 2.5 %). The average nucleotide identity difference values of S5T, SaT and the species reference strains were 92.16-93.62 % and 92.71-93.43%, which confirms that the S5T and SaT represent two novel species of the genera Runella and Dyella, respectively. The draft genome of S5T consisted of 7 048 502 bp, with a DNA G+C content of 44.9 % and that of SaT of 4 398 720 bp with a DNA G+C content of 67.9 %. The phylogenetic, phenotypic and physiological characteristics permitted the distinction of the two strains from their families, and we thus propose the names Runella salmonicolor sp. nov. (type strain S5T = KACC 22689T = TBRC 16343T) and Dyella lutea sp. nov. (type strain SaT=KACC 22690T = TBRC 16344T).