Abstract
Lambs were fed a basal purified diet low in nickel (60 ppb) or the basal diet supplemented with 5ppm of nickel to determine if rumen bacterial urease was a nickel-requiring enzyme. Two collection periods with lambs fed a diet in which all the nitrogen was supplied as preformed protein (casein) indicated that ruminal urease activity was much lower in lambs fed the low nickel diet. When 1% urea was added to the basal diet, urease activity increased slightly with both treatments; however, bacterial urease activity was still much higher in the lambs receiving 5ppm of nickel. Ruminal volatile fatty acids were not influenced by dietary nickel. Ruminal urease requires nickel for maximal activity.
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