Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) experiences several types of post-translational modifications during the expression, import and assembly of the protein. The large subunit (LS) from many plant species contains a trimethyllysyl residue at Lys-14 (1-3). The small subunit (SS) is post-translationally modified by removal of the targeting presequence, and the N-terminal methionine residue subjected to αN-methylation (4). The methylation of Lys-14 in the LS is catalyzed by S-adenosyl-L-methionine (AdoMet): Rubisco LS (lysine) €N-methyltransferase (Rubisco LSMT, Protein Methylase III, EC 2.1.1.127) (5). In spinach a homologue of Rubisco LSMT exists that encodes an enzyme that methylates the SS of Rubisco (Rubisco SSMT). Recombinant tobacco Rubisco LSMT expressed in E. coli and both recombinant and purified pea Rubisco LSMT, also catalyze the methylation of the a amino group of the processed form of the SS.
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