RT-PCR for the identification of developmentally regulated novel members of the kinesin-like superfamily.

Abstract

AbstractThe kinesin-like motor protein superfamily comprises to date about 100 members present in essentially all the major eukaryotic phyla (reviewed in ref. 1), including over 30 in the mouse alone (2). The common feature of the kinesin-like proteins is a highly conserved 350-amino-acid “motor domain” (30–40% sequence identity across species) that includes even more highly conserved ATP- and microtubule-binding sites (3).KeywordsMotor DomainLigation ReactionHybridization TemperatureMixed ProbeLigation EfficiencyThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.