Abstract
Single nucleotide polymorphisms (SNPs) in 3′UTR of key DNA repair enzyme genes are associated with inter‐individual differences of DNA repair capacity (DRC) and susceptibility to a variety of human malignancies such as lung cancer. In this study, seven candidate SNPs in 3′UTR of DRC‐related genes including ERCC1 (rs3212986, rs2336219, and rs735482), OGG1 (rs1052133), MLH3 (rs108621), CD3EAP (rs1007616), and PPP1R13L (rs6966) were analyzed in 300 lung cancer patients and controls from the northeast of China. Furthermore, we introduced ERCC1 (CDS+3′UTR) or CD3EAP (CDS) cDNA clone to transfect HEK293T and 16HBE cells. Cell viability between different genotypes of transfected cells exposed to BPDE was detected by CCK‐8 assay, while DNA damage was visualized using γH2AX immunofluorescence and the modified comet assay. We found that minor A‐allele of rs3212986 could reflect a linkage with increasing risk of NSCLC. Compared with CC genotype, AA genotype of ERCC1 rs3212986 was a high‐risk factor for NSCLC (OR = 3.246; 95%CI: 1.375‐7.663). Particularly stratified by smoking status in cases and controls, A allele of ERCC1 rs3212986 also exhibited an enhanced risk to develop lung cancer in smokers only (P < 0.05). Interestingly, reduced repair efficiency of DNA damage was observed in 293T ERCC1(AA) and 16HBE ERCC1(AA), while no significant difference was appeared in two genotypes of CD3EAP (3′ adjacent gene of ERCC1) overexpressed cells. Our findings suggest that rs3212986 polymorphism in 3ʹUTR of ERCC1 overlapped with CD3EAP may affect the repair of the damage induced by BPDE mainly via regulating ERCC1 expression and become a potential biomarker to predict smoking‐related lung cancer.
Highlights
Lung cancer has high morbidity and mortality and becomes the leading cause of cancer death worldwide
It is reported that the polymorphisms of overlapping gene ERCC1, CD3EAP, and PPP1R13L on the chromosomal region 19q13.3 were associated with individual DNA repair capacity (DRC) and lung cancer susceptibility,[22] which suggested that this exceptional overlapping structure of three genes may have potential functions in carcinogenesis
The present study focuses on the association between lung cancer risk and 3′UTR polymorphisms of DNA repair genes based on a case‐control study
Summary
Lung cancer has high morbidity and mortality and becomes the leading cause of cancer death worldwide. The functional properties based on neighboring SNPs in haplotypes of crucial DNA repair enzymes and DRC‐related proteins as well as the predictive value and the related mechanism of DNA excision repair gene polymorphisms in 3′UTR are still largely uncertainty. In this case‐control study, seven candidate SNPs in 3′UTR of DRC‐related genes, including ERCC1 (rs3212986, rs2336219, and rs735482), OGG1 (rs1052133), MLH3 (rs108621), CD3EAP (rs1007616), and PPP1R13L (rs6966), were analyzed in well‐characterized series of 300 lung cancer patients matched with 300 healthy controls from the northeast of China to prospectively evaluate the associations between DRC‐related polymorphisms in 3′UTR and the risk of lung tumorigenesis. Data from the presented study further indicated that rs3212986 polymorphism in 3′UTR of ERCC1 overlapped with CD3EAP may affect the repair of the damage induced by BPDE mainly via regulating ERCC1 expression and become a potential biomarker to predict smoking‐related lung cancer
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