Abstract
A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of ofloxacin (OFL) and satranidazole (SAT) in combination. The separation is carried out using a mobile phase consisting of 10mM phosphate buffer and methanol in the ratio of 50:50. The pH of the mobile phase is adjusted to 3.0 with 10% o-phosphoric acid. The column used is Kromasil-100 C 18 (250 4.6 mm, 5 µm). with flow rate of 1.0 mL/min using UV detection at 294nm. The total run time is 5 min and the retention time of OFL and SAT is 2.59 min and 4.0 min respectively. The described method is linear for the assay of OFL and SAT over a concentration range of 10‐24 µg/mL and 15‐36 µg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The limit of quantitation for SAT and OFL has been found to be 0.042 µg/mL and 0.085 µg/mL respectively. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of SAT and OFL in bulk drug and its pharmaceutical dosage form.
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