Abstract
This study describes the development of a method for simultaneous analysis of sulfamethoxazole (SMX) and trimethoprim (TMP) through the use of high-performance liquid chromatography/ultraviolet detector, with the application to veterinary medicines. Satisfactory chromatographic separation of SMX and TMP was isocratically with a C18 column (150 x 4.6 mm, 5 mm). A mobile phase consisting of water, pH 3.5, and methanol (60:40, v/v) was delivered at a flow rate of 1.0 mL min-1 for five minutes and then, increased to 1.8 mL min-1. Detection of the drugs was performed at 213 and 230 nm. Linearity was demonstrated in the range of 5 to 70 mg mL-1 for SMX and 1 to 30 mg mL-1 for TMP (r2 ≥ 0.99 for both compounds). The relative standard deviation was ≤ 5%, and the comparison of the results with the concentrations reported on the drug labels indicated that the quantification was accurate. The resultant stressed samples were analysed by the method. The proposed method shows great potential for simultaneous analysis of the drugs evaluated and represents a new alternative approach to quality control of veterinary medicines.
Highlights
Veterinary drugs are used worldwide to improve animal health, provide economic gains and increase food industry productivity of food of animal origin [1]
We developed a method to detect the presence of two drugs extensively used in veterinary clinical practice, sulfamethoxazole and trimethoprim, in a single analysis
SMX and TMP were chromatographically separated in isocratic mode using a reversed phase column and a mobile phase composed by water and methanol (60:40, v/v), delivered at a flow rate of 1.0 mL min-1 for 5 minutes followed by an increase to 1.8 mL min-1
Summary
Veterinary drugs are used worldwide to improve animal health, provide economic gains and increase food industry productivity of food of animal origin [1]. The broad goals of the use of drugs on animals are to preserve the health of the animals, improve animal production and protect public health. Animal health relies heavily on veterinary drugs for controlling pests and diseases, but animal health laws extend much further. The primary concern of many of these laws is the movement of animals and animal products, which can act as vectors for transmission of pests and diseases within and between countries. Such laws typically provide veterinary authorities with strong powers to control animal movement, inspect animals and place them in quarantine, even to destroy infected animals, animal products and equipment. A country’s status as free, or relatively free, of major pests and diseases can have enormous trade benefits, so these laws are usually rigorously enforced by national authorities and scrutinised carefully by international bodies to ensure that they are not used as unfair restraints on trade [1,2]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
