RP – HPLC method for the determination of removal phenol as a pollutant in aqueous solution

Abstract

An efficient, rapid, sensitive, and reproducible methods for the removal phenol determination of in aqueous solution was developed. UV- Spectroscopy methods and reverse phase high performance liquid chromatographic (RP - HPLC) were used. The phenol removal from the aqueous solution was carried out by prepared activated carbons (ACs) from the wheat plant waste as a natural material. The remains phenol was determined using RP – HPLC and UV- Spectroscopy. The elution was done using a mobile phase consisting of acetonitrile (ACN): water in the ratio of 10:90 on HPLC Shimadzu LC–20 A, Japan and Phenomenex C18 column (250 × 4.6 mm, 5μm). The separation was monitored for 15 min at 270 nm using a UV-visible detector and 1.5 ml/min flow rate. UV-VIS spectrophotometric measurement for quantitative of phenol was carried out at 272.6 nm. The optimum conditions such as the composition of the mobile phase, flow rate, temperature, size, time and pH were studied. The obtained results revealed that the values of R2 are (0.9996 and 0.9991), the detection limit (0.02 and 0.01), the quantitative limit (0. 066 and 0.033), the linear ranges (0.2 – 25 and 0.1 – 30) μg / mL for UV and HPLC respectively. The removal percentages of phenol using the treated wheat plant waste were found 98.76 and 98.85 using UV and HPLC measurements respectively. Compared to the other methods, the current method is rapid, simple and economical for the removal and determination of phenol in aqueous solution.