Abstract
RP-HPLC method was developed for simultaneous determination of escitalopram oxalate (ESC) and flupentixol HCl (FLU) in tablet dosage form. Mobile phase consisting of mixture of acetonitrile and potassium phosphate buffer (pH 7.0 with 0.1% triethylamine) in the ratio 60: 40 at flow rate of 1ml/min using C18 Grace (250mmX 4.6mm) column at 231 nm. The retention time of ESC with FLU was found to be 2.96 min and 6.98 min, respectively. The linearity range for ESC with FLU observed was 5-25 µg/ml and 10-50 µg/ml, respectively. Method was validated as per ICH guidelines. Validation parameters studied were linearity and range, recovery study, precision, LOD, LOQ and robustness. Statistical data obtained was found to satisfactorily.
Highlights
Escitalopram oxalate (ESC) is used to treat depression and anxiety
RP-HPLC method was developed for simultaneous determination of escitalopram oxalate (ESC) and flupentixol HCl (FLU) in tablet dosage form
Linearity & range Linearity was studied by injecting a series of dilutions of mixed standard stock solution in the concentration range 20100 μg/ml (ESC) and 1-5 μg/ml (FLU) into the HPLC system using 20μl volume
Summary
It works by helping to restore the balance of a certain natural substance (serotonin) in the brain [1,2] It is official in IP and BP [3,4]. Literature survey revealed various analytical methods have been reported for estimation of ESC alone and in combination with other drugs [28,29,30,31,32,33,34]. In literature there is one UV-spectroscopic method and one RP-HPLC available for simultaneous analysis of ESC and FLU in combined dosage form [37,38]. Attempts were made to develop new RP-HPLC method for simultaneous determination of (ESC) with FLU in tablet dosage form
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