Abstract
In order to more precisely and accurately estimate the antifungal medicine caspofungin and its parenteral dosage form, a new, straightforward, and cost-effective reverse-phase high-performance liquid chromatography (RP-HPLC) technique has been created. The accuracy, precision, linearity, specificity, and reproducibility of the method for the measurement of caspofungin in used equipment were determined during the present study’s validation according to the International Council of Harmonization (ICH) criteria. Elution was accomplished using a CHEMSIL ODS-C18 (5μm) column in isocratic mode with a mobile phase consisting of acetonitrile:ammonium acetate buffer at pH 4.8 (60:40%v/v). The mobile phase was pumped into the column at a flow rate of 1.0 mL/min, and the eluent was detected using a variable wavelength UV detector set at 210 nm. The column dimensions are 250 × 4.6 mm. The chromatogram for the optimized RP-HPLC method of caspofungin estimation had 28,99 theoretical plates (N) and a strong peak at a resolution time of 16,105 minutes. The thorough validation process led to the conclusion that the approach is stable and could be applied for the duration of the drug’s shelf life.
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