Abstract
A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for t he derivative of the Phenyl substituted de rivatives on the hydroxyl prot on of Eugenol . The separation was carried o ut using a mobile phase consisting of 0.5 %v/v Acetic Acid buffer in HPLC grade water (Mobile phase A) and Methanol (Mobile phase B) . The column Iner t sil ODS, 3V ( C 18 , 5 μ, 25 cm × 4.6 mm id with flow rate of 1. 0 ml / min ute using PDA detection at 260 nm. The described method was linear over a concentration range of 10 - 10 0 μg/ml (ppm). All the five mixtures are well separated and the resolution complies as per the pharmacopeia method development requirements . The results of the study showed that the propose d RP - HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantificati ons of the said derivatives of 4 - allyl - 2 - methoxyphenyl 3,4 dimethoxybenzoate (RLS - 228), 4 - allyl - 2 - methoxyphenyl 3,4,5 - trimethoxybenzoate (RLS - 22 2), (Z) - 4 - allyl - 2 - methoxyphenyl 3 - (4 - methoxyphenyl) acrylate (RLS - 205), 4 - allyl - 2 - methoxyphenyl 4 - ethoxy - 3 - methoxybenzoate (RLS - 193) and 4 - allyl - 2 - methoxyphenyl benzoate (RLS - 218)
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