Abstract
A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of benzhexol hydrochloride in tablet formulations and urine. The mobile phase used is acetonitrile and water, ( 50 % v/v ) and the pH was adjusted to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection is at 254nm. The retention time for benzhexol hydrochloride was 7.4 min. The calibration curve was linear up to 40 µg/mL. The mean recovery for benzhexol hydrochloride is 101.16. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.
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