RP-HPLC and UV Method Development for Simultaneous Estimation of Doxofylline, Montelukast and Levocetirizine Dihydrochloride in Pharmaceutical Dosages Form

Abstract

Method has been developed and validated by using spectrophotometric and chromatographic hyphenated techniques for the simultaneous estimation of Doxofylline (DOXO), Montelukast (MONT) and Levocetirizine Dihydrochloride (LEVO) in bulk powder and in pharmaceutical formulations with a high degree of specificity, selectivity and assurances. The first Vierordt’s method was performed and absorption maxima of DOXO, MONT and LEVO at 273.3, 283.1 and 231.05 nm, respectively. The second method employs a first order derivative spectrophotometry (1D) using a crossing technique of measurement at the data point 49, where the measurement was taken at 287.93, 292.57 and 242.45 for DOXO, MONT and LEVO, respectively. Calibration graphs were established in the range of 4-24 μg/mL. RPHPLC method developed by using a C18 (150 x 250 x 4.6 mm) Agilent column and a mobile phase (Ammonia acetate Buffer: ACN) pH 3.5. The retention time of DOXO, MONT and LEVO were found to be 4.425, 7.409 and 8.558 min., respectively. Results of all three methods were found good correlation coefficients. The developed methods have been successfully applied for the determination of ternary or more bulk mixture and formulations dosages forms.