Abstract
Relation between 6-gingerol content and antioxidant activity in in vitro grown cultures of ginger was studied. Reverse phase HPLC analysis revealed that rhizome derived callus culture and micropropagated plants produced lowest amount of 6-gingerol compare to conventionally grown plants. The antioxidant activity of extracts was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Ferric Reducing power assay (FRAP) and correlated with the content of total phenolics and total flavonoids in the extracts. Strong correlation was found between antioxidant activity, total phenolics and 6- gingerol content.
Highlights
The rhizome of ginger (Zingiber officinale, Rosc.) Zingiberaceae has long served culinary and medicinal uses (Afjal et al, 2001)
Using HPLC analysis, callus, micropropagated rhizome and conventionally grown rhizome were investigated for 6-gingerol content (Fig. 1A to 1C)
The extracts of callus and micropropagated plants of ginger proved to be less active in all methods tested
Summary
The rhizome of ginger (Zingiber officinale, Rosc.) Zingiberaceae has long served culinary and medicinal uses (Afjal et al, 2001). Its major pungent constituent, [6]-gingerol has been reported to exhibit antioxidative activity against linoleic acid autoxidation and peroxidation of phospholipid liposomes and to scavenge trichloromethylperoxyl- and 1,1diphenyl-2-picrylhydrazyl (DPPH) radicals (Aeschbach et al, 1994; Sekiwa et al, 2000; Pawar et al, 2011). In addition to these antioxidative effects, our recent study (Ippoushi et al, 2003; Ippoushi et al, 2005) revealed that [6]-gingerol inhibits nitric oxide synthesis in activated J774.1 macrophages and prevents oxidation and nitration reactions induced by peroxynitrite (Radi et al, 2001), a strong reactive nitrogen species. The purpose of this study was to assess the antioxidant activities and to determine 6- gingerol content from the different in vitro ginger extract
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