Rp-diastereomer of adenosine cyclic 3′,5′-phosphorothioate as an antagonist of the stimulatory action of cyclic AMP on the ouabain-insensitive Na efflux in single barnacle muscle fibers

Abstract

Single muscle fibers of the barnacle Balanus nubilus have been used as a preparation to test the possibility that the Rp-diastereomer of adenosine cyclic 3',5'-phosphorothioate, which is the first available analog of cAMP that acts as an antagonist of cAMP, may reduce the magnitude of cAMP-mediated stimulation of the resting ouabain-insensitive Na efflux. The results obtained show that this antagonist is, in fact, able to reduce stimulation of the Na efflux by injected cAMP in a dose-dependent manner.