Roxithromycin decreases ultraviolet B irradiation-induced reactive oxygen intermediates production and apoptosis of keratinocytes

Abstract

Background: In addition to their antimicrobial action, roxithromycin (RXM), a new 14-membrane macrolide antibiotics, have immunomodulatory, anti-inflammatory and anti-oxidative activity. Ultraviolet B (UVB) irradiation induces reactive oxygen intermediates and apoptosis of keratinocytes. Objective : To examine the anti-apoptotic and anti-oxidative effect of RXM on UVB-irradiated keratinocytes. Methods : UVB-induced apoptosis was determined by cell death assay using crystal violet staining, and DNA fragmentation assay. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and calatase activities were measured in UVB-irradiated SV40-trasnformed human keratinocytes (SVHK cells). Detection of superoxide was performed histologically using hydroethidine and colorimetric quantitative assay using ferrous irons. H 2O 2 was measured by colorimetrical assay. Results : RXM suppressed UVB-induced apoptosis of SVHK cells. UVB-irradiated SVHK cells showed decreased SOD, GPx, GR, and catalase activities. RXM pretreatment suppressed the decrease in these enzyme activities with the maximal effect detected at 10 μM of RXM. The effect was associated with suppression of UVB-induced superoxide and H 2O 2 production. Conclusion : The present study demonstrated that RXM has anti-apoptotic and anti-oxidative effects against UVB-irradiated keratinocytes.