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Abstract
From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial applications. Helping substitute for conventional methods such as manual hemocytometry, turbidity, and spectrophotometry, this review presents an image-based, automated cell counter with a fluorescence filter to measure chlorophyll autofluorescence emitted by algae. Capturing chlorophyll-bearing cells selectively, the device accomplished precise qualification of algal numbers. The results for cell density using the device with fluorescence detection were almost identical to those obtained using hemocytometry. The automated functions of the device allow operators to reduce working hours, for not only cell density analysis but simultaneous multiparametric analysis such as cell size and algal status based on chlorophyll integrity. The automated device boldly supports further development of algal application and might contribute to opening up more avenues in the microalgal industry.
Highlights
As is well known, microalgae as primary producers are an important biomass that support freshwater and marine ecosystems
It stands to reason that the quality assurance of microalgae, which are sensitive to environmental changes, is of particular importance [13,14]
In addition to describing general methods for assessing algal quality, this review presents a new method for easy and rapid evaluation of algal numbers and status using a compact, automated, image-based cell counter with a fluorescence filter for measuring chlorophyll autofluorescence
Summary
Microalgae as primary producers are an important biomass that support freshwater and marine ecosystems. In addition to an established role in nature, they provide a high-potential biomass for industrial applications including health and medical areas, food and nutrition science, aquaculture, environmental applications, and chemical production [1,2,3,4,5,6,7,8,9,10,11]. Regardless of the choice of algal species for an application, assessment and maintenance of their quality in culture are critical challenges [11,12]. It stands to reason that the quality assurance of microalgae, which are sensitive to environmental changes, is of particular importance [13,14]. Routine control, maintenance, and management of algal quality in culture is one of the top priorities in industrial applications. In addition to describing general methods for assessing algal quality, this review presents a new method for easy and rapid evaluation of algal numbers and status using a compact, automated, image-based cell counter with a fluorescence filter for measuring chlorophyll autofluorescence
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