Abstract
Mononodal microcuttings with an axillary bud were taken from 2-month-old stock cultures and densely cultured on fresh media in a Petri dish 10 cm×2 cm for different lengths of time, prior to excision of apices. The young apices excised from 12-day-old plantlets produced a conspicuous high level of post-thaw recovery (about 80%) when effectively osmoprotected. The excised apices were precultured on solidified culture medium supplemented with 0.3 M sucrose for 16 h, and then treated with a mixture of 2 M glycerol and 0.4 M sucrose for 20 min. These osmoprotected apices were sufficiently dehydrated with a highly concentrated PVS2 solution for 45 min at 25 °C prior to a plunging into liquid nitrogen. Successfully vitrified apices resumed growth within a week and developed shoots. This vitrification protocol was successfully applied to 10 cultivars of cassava. The average recovery rate amounted to about 70%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
