Abstract
The route of antigen injection is guided by three practical decisions: (1) the volume to be delivered, (2) the buffers and other components to be injected with the immunogen, and (3) how quickly the immunogen should be released into the lymphatics or circulation. For rabbits, large-volume injections normally are given at multiple subcutaneous sites. If adjuvants or particulate matter are included in the injection, the immunogen should not be delivered intravenously. If slow release of the inoculum is desired, the injections should be performed either intramuscularly or intradermally. For immediate release, use intravenous injections.
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