Roundup and immune challenge have different effects on a native field cricket and its introduced competitor.

Abstract

Animals face many natural challenges, and humans have added to this burden by applying potentially harmful herbicides and unintentionally introducing competitors. We examine the recently introduced Velarifictorus micado Japanese burrowing cricket which shares the same microhabitat and mating season as the native Gryllus pennsylvanicus field cricket. In this study, we assess the combined effects of Roundup (glyphosate-based herbicide) and a lipopolysaccharide (LPS) immune challenge on both crickets. In both species, an immune challenge reduced the numbers of eggs that the female laid; however, this effect was much larger in G. pennsylvanicus. Conversely, Roundup caused both species to increase egg production, potentially representing a terminal investment strategy. When exposed to both an immune challenge and herbicide, G. pennsylvanicus fecundity was harmed more than V. micado fecundity. Furthermore, V. micado females laid significantly more eggs than G. pennsylvanicus, suggesting that introduced V. micado may have a competitive edge in fecundity over native G. pennsylvanicus. LPS and Roundup each had differing effects on male G. pennsylvanicus and V. micado calling effort. Overall, introduced male V. micado spent significantly more time calling than native G. pennsylvanicus, which could potentially facilitate the spread of this introduced species. Despite the population-level spread of introduced V. micado, in our study, this species did not outperform native G. pennsylvanicus in tolerating immune and chemical challenge. Although V. micado appears to possess traits that make this introduced species successful in colonizing new habitats, it may be less successful in traits that would allow it to outcompete a native species.