Rough endoplasmic reticulum in the adrenaline and noradrenaline cells of the adrenal medulla: effects of intracranial surgery and pinealectomy.

Abstract

Adrenal medullas in 53-day-old rats of the nonoperated (NO) group (n = 31), the sham-operated (SPX) group (n = 35) and the pinealectomized (PX) group (n = 38) were examined electron microscopically 14 days after surgery. Cell profiles showing solitarily and sparsely distributed rough endoplasmic reticulum (RER) were most frequent in the PX group (daily mean: 66.9%, 427/638), less in the NO (56.0%, 336/600), and least in the SPX (48.5%, 297/612) in adrenaline (A) cells (chi 2-test: P less than 0.001), while most frequent in the NO group (68.8%, 340/494), less in the PX (64.3%, 303/471), and least in the SPX (57.4%, 256/446) (P less than 0.005) in noradrenaline (N) cells. Individual variation was less in A cells than in N cells. Cell profiles showing a large accumulation of RER was more frequent in A cells (NO:8.5%, SPX:13.1%, PX:7.7%) than in N cells (NO:2.8%, SPX:4.5%, PX:4.7%) (controls: P less than 0.001). Sham pinealectomy increased a large accumulation of RER in A cells (P less than 0.02) and a small aggregation of RER in N cells (P less than 0.005) with opposite effects of pinealectomy (P less than 0.005, P less than 0.025). Pinealectomy decreased a small aggregation of RER without effects of sham pinealectomy in A cells (P less than 0.001). (1) Accumulation or aggregation of RER in adrenomedullary chromaffin cells was influenced from the pineal gland either as or without effects of intracranial surgery, and (2) RER in adrenomedullary chromaffin cells showed differences due to cell types.