Abstract
IntroductionIn Sudan, rotavirus has been one of the important causative agents of diarrhea among children. Rotavirus A is well known as the leading cause of diarrhea in young children worldwide. It was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in Sub-Saharan Africa. This study aimed to determine the prevalence and the common clinical presentations of rotavirus A infection among Sudanese children with gastroenteritis seeking management in hospitals.Methods755 Sudanese children less than 5 years of age suffering from acute gastroenteritis in hospital settings were included. The positive stool specimens for rotavirus A was used for extract Ribonucleic acid (RNA) and the RNA product was loaded on formaldehyde agarose gel and visualized under UV illumination.ResultsOf the 755 children, 430(57%) were males while 325(43%) were female. The age of children ranged from 1 to 60 months. There were 631 (84%) children who were less than 24 months of age. Out of the 755 stool samples, 121(16%) were positive for rotavirus. Of the 121 infected children with rotavirus, 79(65.3%) were male and 42(34.7%) were female and the highest infection rate was seen among 91(75.2%) of children up to 12 months of age. Children of illiterate parents were more infected with rotavirus than children of educated parents. Severe dehydration present among 70% of infected children with rotavirus.ConclusionSince this study is hospital-based, the 16% prevalence rate may not reflect the true prevalence among Sudanese children, thus a community-based surveillance is needed.
Highlights
In Sudan, rotavirus has been one of the important causative agents of diarrhea among children
Formaldehyde Agarose (FA) gel electrophoresis of prepared Ribonucleic acid (RNA): Formaldehyde agarose gel electrophoresis was employed for those prepared RNA samples as following: 5?l of 5× RNA loading buffer were added to 20?l of each RNA sample mixed and incubated for 3-5 min at 65°C chilled on ice
The prevalence rate of rotavirus infection among children with gastroenteritis in this study is 16% and the infection rate is higher in males than females
Summary
In Sudan, rotavirus has been one of the important causative agents of diarrhea among children. Rotavirus A is well known as the leading cause of diarrhea in young children worldwide. It was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in Sub-Saharan Africa. Methods: 755 Sudanese children less than 5 years of age suffering from acute gastroenteritis in hospital settings were included. The positive stool specimens for rotavirus A was used for extract Ribonucleic acid (RNA) and the RNA product was loaded on formaldehyde agarose gel and visualized under UV illumination. The World Health Organization attributed a worldwide estimate of 17% mortality due to diarrhea in children younger than 5 years of age, 40% of them were in Africa [1]. Rotavirus estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in Sub-Saharan Africa [3]. Rotaviruses are members of the Reoviridae family can possess a genome of 11 segments of double stranded Ribonucleic acid (dsRNA) that encode six viral structural proteins and six nonstructural proteins [4]
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