Abstract
The rotational correlation times of the natural flavin compounds riboflavin and FMN and the flavin models lumiflavin, tetra-O-acetyl-riboflavin and their N (3)-methylated derivatives, dissolved in water, were determined with the time-resolved fluorescence depolarization technique. Owing to the time resolution the effect of methyl group substitution could be determined as a few picoseconds longer correlation time, in agreement with the slightly larger molecular volume. Anisotropic rotation of lumiflavin was demonstrated by observing the change in correlation time when excitation was in the second excited singlet state of the flavin. The angle between the emission transition moment and the long axis of the flavin ring system is approximately 22°.
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