Abstract
Establishment of left‐right sidedness is a crucial early event in vertebrate morphogenesis. It is currently believed that left‐right asymmetry patterns result from genetic regulatory networks via extracellular morphogens and/or ciliary activity in all vertebrates. However the role of biomechanical motion near the organizer region, has been overlooked. Electroporation of histone 2B‐YFP nuclear marker into avian nodal epiblastic cells and time‐lapse imaging (HH 4‐6) revealed the movements of labeled cells during the interval when morphological and gene expression asymmetries are first observed. The recordings indicated that relative motion occurs between the organizer (Hensen's node) and the surrounding epiblastic epithelium in avians. Specifically, an asymmetric counterclockwise rotation of epiblastic nodal tissue was observed following electroporation on the embryonic left side of the node. However, on the embryonic right, a two‐pronged migratory path was observed. The rotational motion occurs during the interval when initial expression of Shh and Fgf8 is symmetrical, and continues until establishment of Shh and Fgf8 left‐right asymmetry. Ongoing work is aimed at influencing the biomechanical tissue motion and analysis of resulting gene expression patterns. Supported by an AHA Fellowship 0620105Z, the NIH P20 RR016475 from the INBRE Program, NCRR; NIHLB R01 068855; and the Mathers Foundation.
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